DIDS and the Janus-faced Na+-K+-activated ATPase. Focus on “DIDS inhibits Na-K-ATPase activity in porcine nonpigmented ciliary epithelial cells by a Src family kinase-dependent mechanism”
نویسندگان
چکیده
منابع مشابه
DIDS inhibits Na-K-ATPase activity in porcine nonpigmented ciliary epithelial cells by a Src family kinase-dependent mechanism.
The anion transport inhibitor DIDS is known to reduce aqueous humor secretion but questions remain about anion dependence of the effect. In some tissues, DIDS is reported to cause Na-K-ATPase inhibition. Here, we report on the ability of DIDS to inhibit Na-K-ATPase activity in nonpigmented ciliary epithelium (NPE) and investigate the underlying mechanism. Porcine NPE cells were cultured to conf...
متن کاملDIDS and the Janus-faced Na⁺-K⁺-activated ATPase. Focus on "DIDS inhibits Na-K-ATPase activity in porcine nonpigmented ciliary epithelial cells by a Src family kinase-dependent mechanism".
THE MAINTENANCE OF MAMMALIAN cell volume fundamentally rests on net cation extrusion through Na -K -activated ATPase, the Na pump. The publication by Shahidullah et al. (11) persuasively identifies the series of events by which a widely used inhibitor of anion transport, DIDS, can reduce Na pump activity of porcine nonpigmented ciliary epithelial (NPE) cells. DIDS sequentially acidifies the NPE...
متن کاملNa,K-ATPase and epithelial tight junctions.
Tight junctions are unique organelles in polarized epithelial and endothelial cells that regulate the flow of solutes and ions across the epithelial barrier. The structure and functions of tight junctions are regulated by a wide variety of signaling and molecular mechanisms. Several recent studies in mammals, drosophila, and zebrafish reported a new role for Na,K-ATPase, a well-studied ion tran...
متن کاملMultiple functions of Na,K-ATPase in epithelial cells.
The Na,K-adenosine triphosphatase (ATPase), or sodium pump, has been well studied for its role in the regulation of ion homeostasis in mammalian cells. Recent studies suggest that Na,K-ATPase might have multiple functions such as a role in the regulation of tight junction structure and function, induction of polarity, regulation of actin dynamics, control of cell movement, and cell signaling. T...
متن کاملSGK1 activates Na+-K+-ATPase in amphibian renal epithelial cells.
Serum- and glucocorticoid-induced kinase 1 (SGK1) is thought to be an important regulator of Na(+) reabsorption in the kidney. It has been proposed that SGK1 mediates the effects of aldosterone on transepithelial Na(+) transport. Previous studies have shown that SGK1 increases Na(+) transport and epithelial Na(+) channel (ENaC) activity in the apical membrane of renal epithelial cells. SGK1 has...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: American Journal of Physiology-Cell Physiology
سال: 2013
ISSN: 0363-6143,1522-1563
DOI: 10.1152/ajpcell.00114.2013